Best of 50 Cent anthologizes the majority of the singles Curtis Jackson released during his joint deals with Eminem's Shady Records, Dr. Dre's Aftermath, and major label Interscope. From "In da Club" through "Ayo Technology," all of the Top Ten pop hits are here, as are most of the other A-sides from Get Rich or Die Tryin', The Massacre, the Get Rich soundtrack, Curtis, and Before I Self Destruct. The relatively minor non-album 2008-2009 singles "Get Up" and "I Get It In" are part of the program. "How to Rob," the circa-1999 pre-supernova cut Jackson contributed to the Sony-distributed In Too Deep soundtrack (also intended for the canceled Power of the Dollar), is added as a bonus. This provides a representative summary of the most commercial recordings by one of the decade's most popular and polarizing artists.
| A1 | In Da Club | 3:14 |
| A2 | 21 Questions | 3:44 |
| A3 | P.I.M.P. | 4:10 |
| A4 | Disco Inferno | 3:34 |
| B1 | Candy Shop | 3:27 |
| B2 | Just A Lil Bit | 3:58 |
| B3 | Outta Control (Remix) | 4:14 |
| B4 | Hustler's Ambition | 3:57 |
| C1 | Best Friend (Remix) | 4:14 |
| C2 | Window Shopper | 3:10 |
| C3 | Ayo Technology | 4:08 |
| C4 | I Get Money | 3:44 |
| C5 | Straight To The Bank | 3:11 |
| D1 | I'll Still Kill | 3:44 |
| D2 | Get Up | 3:14 |
| D3 | I Get It In | 3:20 |
| D4 | Baby By Me | 3:33 |
| D5 | How To Rob | 4:24 |
